TABLE 2.
Real-time PCR analysis of live versus gamma-irradiated B. anthracis spores using the BA3 chromosomal primer/probe set
| Strain IDa | Avg CT (mean ± SD)b
|
|
|---|---|---|
| Live spores | Irradiated spores | |
| K1340 | 21.1 ± 0.57 | 18.3 ± 0.53 |
| K9002 | 22.3 ± 0.22 | 18.9 ± 0.21 |
| K5135 | 24.9 ± 0.21 | 19.1 ± 0.31 |
| K2484 | 25.3 ± 0.33 | 19.4 ± 0.51 |
| K1694 (Ames) | 24.3 ± 0.54 | 19.9 ± 0.30 |
| K4596 (Vollum) | 25.5 ± 0.34 | 19.0 ± 0.30 |
| K2762 | 22.0 ± 0.57 | 18.7 ± 0.48 |
| K2478 | 21.6 ± 0.39 | 19.3 ± 0.27 |
a
ID, identification.
b
The differences in CT values for live (untreated) versus irradiated spores were found to be significant by paired t test (two tailed) (P = 0.001; n = 24).