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GA-resistant transformants in which the MaNPS1 gene was disrupted (KO B1-3, KO 8-18, KO 37-2, and KO 43-1) exhibited a 3.7-kb amplicon after PCR with primer pair Sma267F and 2EndR, whereas the wild-type strain ARSEF 2575 and the ectopic transformant (A-18-1) had a 1.9-kb amplicon representative of the intact gene without the Bar expression cassette (1.8 kb in size) (Fig. 1B).
