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Southern analysis of SacI-digested genomic DNA of ΔManps1 mutants and control strains. The membrane was probed first with a DIG-labeled fragment of clone Ma#267 and then stripped and probed with a DIG-labeled fragment of the Bar gene. WT, ARSEF 2575; A18, Ect A18-1; B1-3, KO-B1-3; 8-18, KO 8-18; 37-2, KO 37-2; 43-1, KO 43-1. Select size markers from a 1-kb DNA ladder (Promega) are indicated on the left side of the figure.
