TABLE 5.
Mean concentrations of enterobactin-related molecules detected in culture supernatants
| Strain (genotype) | Concn (μM) of enterobactin-related moleculea:
|
|||
|---|---|---|---|---|
| Enterobactin | (DHBS)3 | (DHBS)2 | DHBS | |
| χ7122 | 12.1 ± 3.4 | 25.3 ± 4.6 | 33.1 ± 4.7 | 11.6 ± 1.2 |
| χ7304b | 62.9 ± 7.2 | 62.7 ± 6.2 | 86.6 ± 9.3 | 25.9 ± 3.9 |
| χ7304(pYA3661 iroBCDEN)b | 1.5 ± 0.16 | 1.9 ± 0.25 | 9.6 ± 1.01 | 27.1 ± 3.7 |
| χ7304(pIJ34 iroBCDE)c | 0.9 ± 0.07 | 0.9 ± 0.11 | 10.9 ± 1.9 | 20.8 ± 3.1 |
| χ7304(pIJ137 iroBCDN)c | 3.8 ± 0.14 | 5.1 ± 0.23 | 22.2 ± 0.9 | 13.9 ± 0.8 |
| χ7304(pIJ136 iroBCEN)c | 0.6 ± 0.06 | 0.8 ± 0.12 | 8.6 ± 0.57 | 15.0 ± 1.3 |
| χ7304(pIJ53 iroB)c | 1.6 ± 0.35 | 0.6 ± 0.10 | 0.5 ± 0.01 | ND |
| χ7304(pIJ121 iroBC)c | 3.7 ± 0.20 | 2.9 ± 0.09 | 10.7 ± 0.4 | 1.6 ± 0.10 |
| χ7304(pIJ135 iroBCN)c | 4.6 ± 0.36 | 3.9 ± 0.29 | 11.8 ± 0.5 | 2.6 ± 0.15 |
| χ7304(pIJ37 iroBDEN)c | 2.7 ± 0.25 | 3.7 ± 0.35 | 15.3 ± 1.5 | 8.2 ± 1.12 |
a
Values represent means ± the standard errors of the means of at least three independently grown cultures. Bold values indicate significant differences (P < 0.05) using a two-tailed unpaired t test. ND, molecule not detected in samples.
b
Statistical differences were determined by using χ7122 as the reference strain.
c
Statistical differences were determined by using χ7304(pYA3661 iroBCDEN) as the reference strain.