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. 2008 May 27;76(8):3464–3472. doi: 10.1128/IAI.00037-08

FIG. 5.

FIG. 5.

Delayed M. tuberculosis antigen-specific T-cell response in ApoE−/− HC mice. (A) Amounts of IFN-γ released by splenocytes 10 days, 20 days, and 30 days after aerosol infection with M. tuberculosis from WT HC and ApoE−/− HC mice that were cultured for 48 h with 2 μg/ml M. tuberculosis Erdman CFP. Individual measurements are shown, and the mean concentration is indicated with a solid line. Day 10 was below the lower limit of detection of 31 pg/ml. *, P < 0.05 (n = 5). (B) Splenocyte counts from WT HC and ApoE−/− HC mice 10 days, 20 days, and 30 days after aerosol infection with M. tuberculosis. The values are the mean splenocyte counts plus standard deviations (SD). *, P < 0.05 (n = 5). (C) The total numbers (top graphs) and percentages (bottom graphs) of T cells (CD3+ CD4+/CD8+), macrophages (F4/80+), or granulocytes (Gr-1+ CD3− F4/80−) isolated from the spleens of WT HC and ApoE−/− HC mice 10 days, 20 days, and 30 days after aerosol infection with M. tuberculosis. The total number of each cell population was determined by multiplying the percentage of cells positive for the above-mentioned surface markers by the total number of splenocytes isolated from each mouse. The values are the mean numbers of cells expressed in millions plus SD. *, P < 0.05 (n = 4). The results shown for panels A and B are representative of two experiments, and the results for panel C are representative of one experiment.