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. 2008 Jun 9;76(8):3577–3586. doi: 10.1128/IAI.00269-08

FIG. 5.

FIG. 5.

TNF-α, IL-1β, and CINC-1 cell-free BALF concentrations from rats at 3, 6, 24, 48, 72, and 168 h post-challenge with different titers of A. baumannii strain 307-0294. (A) TNF-α. (B) IL-1β. (C) CINC-1. Rats were given 7.0 × 106, 5.8 × 107, 3.5 × 108, 4.3 × 108, and 7.7 × 109 CFU of 307-0294 (blood isolate, ST15, clonal group 1) by intratracheal instillation, and BALF was obtained for determination of cytokine/chemokine levels at 3, 6, 24, 48, 72, and 168 h. (A) There was a significant decrease in TNF-α levels at 6 h in rats challenged with 7.0 × 106 CFU compared to rats challenged with 4.3 × 108 CFU (*, P = 0.01) and a trend for decreased TNF-α levels at 3 h in rats challenged with 7.0 × 106 CFU compared to rats challenged with 7.7 × 109 CFU (&, P = 0.049), at 6 h in rats challenged with 7.0 × 106 CFU compared to rats challenged with 3.5 × 108 and 7.7 × 109 CFU (&, P = 0.02), at 6 h in rats challenged with 5.8 × 107 CFU compared to rats challenged with 4.3 × 108 CFU (&, P = 0.04), and at 24 h in rats challenged with 7.0 × 106 CFU compared to rats challenged with 3.5 × 108 CFU (&, P = 0.04). (B) There was a trend for decreased IL-1β levels at 24 h in rats challenged with 7.0 × 106 CFU compared to rats challenged with 3.5 × 108 CFU (&, P = 0.04). (C) There was a significant decrease in CINC-1 levels at 3 h in rats challenged with 7.0 × 106 CFU compared to rats challenged with 7.7 × 109 CFU (*, P = 0.007) and at 6 h in rats challenged with 7.0 × 106 compared to rats challenged with 3.5 × 108, 4.3 × 108, and 7.7 × 109 CFU (*, P = 0.004, 0.0003, and 0.004, respectively) and in rats challenged with 5.8 × 107 CFU compared to rats challenged with 3.5 × 108, 4.3 × 108, and 7.7 × 109 CFU (*, P = 0.007, 0.0006, and 0.008, respectively). Data are means ± standard errors of the means for n = 3 for each challenge titer and time point.