Skip to main content
. 2008 Jun 9;76(8):3577–3586. doi: 10.1128/IAI.00269-08

FIG. 6.

FIG. 6.

Neutrophil numbers in BALF from rats at 3, 6, 24, 48, 72, and 168 h post-challenge with different titers of A. baumannii strain 307-0294. Rats were given 7.0 × 106, 5.8 × 107, 3.5 × 108, 4.3 × 108, and 7.7 × 109 CFU of 307-0294 by intratracheal instillation; BALF was collected at 3, 6, 24, 48, 72, and 168 h; and cells were harvested from BALF and then enumerated by Coulter counting. Neutrophil numbers are based on leukocyte differentials obtained from stained cytoslides (Materials and Methods). Cell counts were not done from animals challenged with 7.7 × 109 CFU. There was a significant decrease in the number of neutrophils at 72 h in rats challenged with 7.0 × 106 CFU compared to rats challenged with 4.3 × 108 CFU (*, P = 0.0003) and a trend for a decrease in the number of neutrophils at 24 h in rats challenged with 7.0 × 106 CFU compared to rats challenged with 5.7 × 107 and 4.3 × 108 CFU (&, P = 0.019 and 0.041, respectively), at 48 h in rats challenged with 7.0 × 106 CFU compared to rats challenged with 3.5 × 108 and 4.3 × 108 CFU (&, P = 0.025 and 0.019, respectively), at 72 h in rats challenged with 7.0 × 106 CFU compared to rats challenged with 3.5 × 108 CFU (&, P = 0.037), and at 168 h in rats challenged with 7.0 × 106 CFU compared to rats challenged with 5.7 × 107 and 4.3 × 108 CFU (&, P = 0.022 and 0.022, respectively). Data are means ± standard errors of the means for n = 3 for each challenge titer and time point.