TABLE 3.
Microarray and real-time RT-PCR validation of ΔccpA strain compared to MGAS5005
| 5005_ Spy no.d | Annotation (TIGR or NCBI) | Gene | Array mean ± SEa | RT-PCR mean ± SEe | Presence of cre |
|---|---|---|---|---|---|
| Spy1275 | Arginine deiminaseb | arcA | 0.027 ± 0.02 | 0.044 ± 0.01 | Yes |
| Spy1065 | α-Cyclodextrin glycosyltransferase | amyA | 0.03 ± 0.01 | 0.073 ± 0.01 | No |
| Spy0475 | PTS system, β-glucoside-specific II ABC | bglP | 0.038 ± 0.01 | 0.071 ± 0.02 | No |
| Spy0562 | SLS precursor; pel locusb | sagA | 0.039 ± 0.01 | 0.052 ± 0.01 | Yes |
| Spy1067 | Maltose ABC transporter, periplasmic binding | malX | 0.077 ± 0.03 | 0.05 ± 0.02 | No |
| Spy0780 | PTS system, mannose/fructose family IIAb | ptsA | 0.128 ± 0.08 | 0.124 ± 0.04 | Yes |
| Spy1746 | PTS system, cellobiose-specific IIA | celC | 0.148 ± 0.05 | 0.759 ± 0.33 | Yes |
| Spy1381 | Glycerol kinase | gplK | 0.192 ± 0.11 | NT | Yes |
| Spy0127 | ATP synthase, subunit Kb | ntpK | 0.209 ± 0.17 | NT | Yes |
| Spy1305 | Two-component response regulator TCS-10 | tcs10R | 0.275 ± 0.28 | 0.365 ± 0.18 | Yesc |
| Spy1479 | PTS system mannose-specific IIABb | manL | 0.361 ± 0.09 | NT | Yes |
| Spy0926 | Cardiolipin synthase, putativeb | 0.408 ± 0.10 | NT | Yes | |
| Spy1635 | Tagatose 1,6-diphosphate aldolase | lacD2 | 0.432 ± 0.31 | NT | No |
| Spy0785 | Two-component response regulator YesNM | tcs5R | 0.445 ± 0.08 | NT | Yesc |
| Spy1738 | Secreted DNase; streptodornase B; mitogenic factor | spd | 0.46 ± 0.10 | NT | No |
| SPy0141 | Streptolysin O precursor | slo | 1.025 ± 0.13 | 1.076 ± 0.21 | No |
| Spy1720 | Multigene regulator of virulence (Mga) | mga | 1.087 ± 0.14 | 1.024 ± 0.06 | Yes |
| Spy0283 | Two-component sensor kinase; virulence associated | covS | 1.187 ± 0.16 | 1.881 ± 0.63 | No |
| Spy1851 | Hyaluronate synthase; capsule synthesis | hasA | 1.189 ± 0.13 | 1.889 ± 0.31 | No |
| Spy0106 | RofA; stand-alone virulence regulator RofA | rofA | 1.26 ± 0.17 | 1.536 ± 0.35 | No |
| Spy0186 | RofA-like protein RALP-4; virulence regulator | rivR | 1.983 ± 0.32 | 2.223 ± 0.29 | Yes |
| Spy0988 | Pyruvate kinase | pyk | 2.345 ± 0.22 | 3.154 ± 0.64 | No |
| Spy0576 | ATP synthase subunit 6b | atpB | 2.349 ± 0.30 | 2.347 ± 0.27 | No |
| Spy0424 | Catabolite control protein A (CcpA) | ccpA | 4.615 ± 3.05 | 1261 ± 105 | Yes |
a
Data are sorted by array mean values.
b
First gene in CcpA-regulated operon.
c
Associated with cre present at beginning of operon.
d
Genes shown in bold indicate CcpA repression, and underlined genes show activation.
e
NT, not tested.