TABLE 1.
Protein | Antigen | Oligomerization domain | Elution vol (ml) |
---|---|---|---|
IMX183 | MSP119 | None | |
IMX269 | MSP119 | None | |
IMX108 | MSP119 | Murine C4bp | 163 |
IMX180 | MSP119 | Human C4bp | 164 |
IMX272 | MSP119 | Archaeal Sm | 152 |
IMX262 | MSP119 | Chicken2 C4bp | 165 |
IMX314 | MSP119 | Chicken1 C4bp | |
IMX317 | MSP119 | Chicken2 C4bpΔ7 | 161 |
IMX313 | MSP119 | Hybrid C4bpΔ7 | 162 |
IMX63 | DsbA | None | |
IMX253 | DsbA | Murine C4bp | |
IMX259 | IF tag | Chicken2 C4bp | |
IMX117 | IF tag | Human C4bp | |
IMX273 | IF tag | Archaeal Sm | |
IMX315 | None | Chicken2 C4bp |
Recombinant proteins used in this study were all prepared from E. coli. The IF tag functions like a histidine tag and facilitates purification; however, the murine C4bp domain was not well expressed using this tag, and it was replaced by the DsbA protein.