TABLE 2.

Increasing the dose of IMX108 increases the antibody response^a

Antigen	Dose (μg)	Dose (nmol)	IFA titer	ELISA titer
IMX108	5	0.24	1/10,240	1/102,400
	10	0.48	1/10,240	1/102,400
	20	0.95	1/10,240	1/102,400
	40	1.9	1/20,480	1/204,800
	80	3.8	1/20,480	1/204,800
	140	6.7	1/40,960	1/409,600
	200	9.5	1/40,960	1/409,600
IMX183	23	1.9	1/80	<100

^aGroups of three BALB/c mice were immunized subcutaneously three times at 4-week intervals with different amounts of the IMX108 protein (MSP1₁₉-murine C4bp) without additional adjuvant. Control mice received IMX183 (MSP1₁₉ alone). Antibody titers were determined by IFA or by ELISA using pooled serum samples collected from each group 3 weeks after the third immunization. The IFA provided a measure of the amount of antibody binding to native MSP1 on the parasite, whereas the ELISA provided a measure of the amount of antibody binding to the recombinant MSP1₁₉.