TABLE 3.
Antibody titers against P. yoelii MSP119 induced by different oligomerization domainsa
| Immunogen(s) | Oligomerization domain | Freund's adjuvant | Titer with coated antigen
|
||||
|---|---|---|---|---|---|---|---|
| P. yoelii Msp119b | Murine C4bp | Human C4bp | Chicken2 C4bp | Archaeal Sm | |||
| IMX269 | None | − | <100 (0) | ||||
| IMX269 + IMX315 | Chicken2 C4bp | − | <100 (0) | ||||
| IMX269 | None | + | 3,200 (7,390) | ||||
| IMX272 | Archeal Sm | − | 12,800 (6,350) | <100 | <100 | 102,400 | |
| IMX180 | Human C4bp | − | 12,800 (13,418) | <100 | 6,400 | <100 | |
| IMX180 | Human C4bp | + | 51,200 (51,731) | ||||
| IMX108 | Murine C4bp | − | 25,600 (9,776) | ||||
| IMX108 | Murine C4bp | − | 25,600 (9,776) | 3,200 | <100 | ||
| IMX108 | Murine C4bp | − | 51,200 (28,637) | 3,200 | <100 | <100 | <100 |
| IMX108 | Murine C4bp | + | 102,400 (90,000) | 3,200 | <100 | <100 | <100 |
| IMX314 | Chicken1 C4bp | − | 51,200 (29,560) | ||||
| IMX262 | Chicken2 C4bp | − | 102,400 (0) | <100 | 12,800 | ||
| IMX262 | Chicken2 C4bp | − | 102,400 (48,036) | ||||
| IMX262 | Chicken2 C4bp | − | 102,400 (78,209) | ||||
| IMX317 | Chicken2 C4bpΔ7 | − | 102,400 (103,467) | ||||
| IMX313 | Hybrid C4bpΔ7 | − | 204,800 (0) | ||||
| IMX313 | Hybrid C4bpΔ7 | − | 204,800 (0) | ||||
a
Groups of three BALB/c mice were immunized as described in Materials and Methods. Antibody titers were determined by ELISA in serum samples collected 2 weeks after the second immunization (week 6). Three different lots of IMX108 and IMX262 and two different lots of IMX313 were tested, and the results for each test are shown on a separate line. If there is no entry, no test was done.
b
The values in parentheses are standard deviations.