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Differential activation of hTLR4/hMD-2 by H44/76 and LpxL1. HeLa 57A cells transfected with hTLR4, hMD-2, and hCD14 were stimulated with purified H44/76 LPS or LpxL1 LPS (A) or PFA-fixed N. meningitidis H44/76 or the LpxL1 mutant (B) at the indicated concentrations. After 5 h, NF-κB luciferase activities, indicated in RLU, were measured. The values are the means ± SEMs of at least three experiments.
