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Activation of mTLR4/mMD-2 by H44/76 and the LpxL1 mutant. HeLa 57A cells transfected with mTLR4, mMD-2, and mCD14 were stimulated with purified H44/76 LPS or LpxL1 LPS (A) or PFA-fixed N. meningitidis H44/76 or the LpxL1 mutant (B) at the indicated concentrations. After 5 h, NF-κB luciferase activities were measured. Values are expressed in RLU and represent the means ± SEMs for the results of at least three experiments.
