FIG. 5.

Association/invasion/survival assays performed using wild-type (WT) N. gonorrhoeae 1291, 1291 mntAB, and 1291 mntC with 90 min of infection without (association) or with (invasion) 30 min of gentamicin treatment (100 μg/ml) and then a 1-h or 3-h second incubation (survival) in antibiotic-free medium. Gonococcal association, invasion, and survival within pex cells was determined in parallel in a single assay; however, the data obtained have been plotted as individual panels. Data shown are the means (variances) obtained from three separate assays performed in triplicate.