TABLE 2.
Primers used in this study
| Gene | Primer | Sequence (5′→3′) |
|---|---|---|
| PCR target genes | ||
| ltxA | ltxAup | GCCCGGGATGGCAACTACTACACTGCTAAATAC |
| ltxAdown | GCCCGGGCAGTAGTTGCTAACGAATTTGC | |
| ltxB | ltxBup | CGCAAATTCGTTAGCAACTACTGC |
| ltxBdown | CAAGTTTTCATTATCGTTCGTTCC | |
| ltxC | ltxCup | AAAAACTATTGGAATACCAAGTAC |
| ltxCdown | GTAGTAGTTGCCATAATCTATTCTC | |
| ltxD | ltxDup | ATTACAAGTAAATTAAAGGAACGAAC |
| ltxDdown | GAAAACCGGAATGTTATATTCTATC | |
| tdeA | tdeAup | CGCCATGGGGCGTGCCGCATGCG |
| tdeAdown | CCGATTACAGCGTTGGCGCAAG | |
| ptsH | ptsHup | TCCTGAAATCATAGTAACCTTC |
| ptsHdown | GGTTTGTGGCATCTGGAAGATG | |
| ptsI | ptsIup | GCGGATCCCAAGGAGATTTAATATGATTTCAGGAATTCCGGC |
| ptsIdown | CGCAAGCTTCTAATTTAATGCTTTTTCATAC | |
| RT-PCR target genesa | ||
| ltxA | ltxA-s | GTGCTAGGTAAACATCGGTAAAG |
| ltxA-as | GACCACAGAGGCAATTAACC | |
| glyA | glyA-s | CCCAATTCACCAACAAATATGC |
| glyA-as | ATTCTTTCGCACGCTCAATAG |
a
RT-PCR, real-time PCR.