TABLE 3.
PCR mapping of LtxA mutants
| Strain | Hemolysis | Relative product size after PCR amplification of the indicated genea
|
||||
|---|---|---|---|---|---|---|
| ltxC | ltxA | ltxB | ltxD | tdeA | ||
| MP07 | +/− | WT | WT | WT | WT | Mut |
| MP028 | − | WT | WT | Mut | WT | WT |
| MP035 | − | WT | Mut | WT | WT | WT |
| MP038 | − | WT | Mut | WT | WT | WT |
| MP040 | − | WT | WT | Mut | Mut | WT |
| MP041 | +/− | WT | WT | WT | WT | Mut |
| MP042 | − | WT | Mut | WT | WT | WT |
| MP043 | − | WT | Mut | WT | WT | WT |
| MP046 | +/− | WT | WT | WT | WT | WT |
| MP047 | − | WT | WT | WT | WT | Mut |
a
WT, product size equal to that of the wild type; Mut, increase in the product size of approximately 1 kbp.