FIG. 1.

Comparative in vitro and in vivo expression levels of stuA, sspA, hsp70, and ura7 determined by real-time RT-PCR. RNA in vitro was derived from cultures that were grown for 4 h, 8 h, and 1 to 7 days at 37°C in YPD medium. For in vivo data, mice were infected with wild-type strain Af293 in an inhalational chamber (A) or intranasally (B). At the indicated time points, infected lungs were removed, and total RNA was then used for real-time RT-PCR analysis (n = 3 per time point). The expression levels of all genes were normalized to the expression level of the endogenous control gene TEF1. The expression value at 4 h for each gene was used as the baseline. Arrows indicate the onset of developmental competence in vitro. n.d. indicates that fungal RNA was not detected at this time point.