FIG. 3.

Mycotoxin expression is induced in vivo. Comparative in vitro and in vivo expression levels of gliP, aspHS, aspF1, metAP1, dmaW, and mitF were determined by real-time RT-PCR. RNA in vitro was derived from cultures that were grown for 5 to 8 days at 37°C in YPD medium. For in vivo data, mice were infected with wild-type strain Af293 in an inhalational chamber. On days 5 to 8 after infection, the infected lungs were removed, and total RNA was then used for real-time RT-PCR analysis (n = 3 mice per time point, with 12 mice total). Results from all four time points in vivo were very similar and were combined. Average expression values for each gene were normalized to values for TEF1 expression. ★ indicates statistically different results from in vitro expression by log-rank test (P < 0.05).