FIG. 4.

Comparative in vitro and in vivo expression levels of gliP determined by real-time RT-PCR. Expression values are normalized to TEF1 expression values. (A) RNA in vitro was derived from cultures of wild-type strain Af293, the ΔstuA mutant, or the stuA-complemented strain, which were grown for 24 h and 72 h at 37°C in YPD or RPMI medium. Note that data for stuA expression in YPD and in vivo from Fig. 2 are used for comparative purposes. (B) Mice were infected by intranasal instillation of wild-type strain Af293, the ΔstuA mutant, or the stuA-complemented strain. Twenty-four hours and 72 h after infection, infected lungs were removed for total RNA extraction, and the mRNA levels of gliP were determined by real-time RT-PCR. All experiments were repeated in triplicate on at least two separate occasions.