TABLE 1.
Genes tested in this study and the primers used in real-time RT-PCR assays
| Gene | Oligonucleotide sequence (5′-3′)a | Amplicon size (bp) | Gene accession no. or identifier (source or reference) |
|---|---|---|---|
| tef1 | CCATGTGTGTCGAGTCCTTC (F) | 84 | Afu1g06390 |
| GAACGTACAGCAACAGTCTGG (R) | |||
| sspA | GACCGTCACTCTGACCTCAA (F) | 71 | Afu8g03930 (this work) |
| CTGTGGGAAGGGTAGTGCTT (R) | |||
| stuA | GAGGACGAAGGGAGTCTCTG (F) | 83 | Afu2g07900 (21) |
| ACCGTTGATCATGTGGTTGT (R) | |||
| hsp70 | TGTCATCACCGTACCAGCTT (F) | 93 | Afu8g03930 (this work) |
| TGATGATGCGGAGAACATTT (R) | |||
| ura7 | CATCTTCGGCTCACAAGAGA (F) | 113 | Afu2g03930 (this work) |
| CCAAAGTGAACATCCGATTG (R) | |||
| gliP | TCCAACAGTCAGAGGCATTC (F) | 103 | AY838877 (13) |
| CTTGAGGGATAATCGGTGGT (R) | |||
| aspF1 | TACCCGCACTGGTTCACTAA (F) | 94 | M83781 (2) |
| GACGGTCACAGTCGGCTT (R) | |||
| 18S rRNA | GGCCCTTAAATAGCCCGGT (F) | 62 | AB008401 (6) |
| TGAGCCGATAGTCCCCCTAA (R) | |||
| mitF | AGCCGTGTCTGTTCTAGCTG (F) | 72 | X58278 (16) |
| AGCTGTTGGTTGATGCATGT (R) | |||
| dmaW | GAATGGTTCTTACCGGGTTG (F) | 118 | XM_749235 (8) |
| GTGTTGAATGGGTCTTGTGC (R) | |||
| metAP | GTTGTCCACGCTACGGAGTA (F) | 132 | Afu8g00410 (this work) |
| CGGTGATTCTCAGCTTCTCA (R) | |||
| aspHS | AGTCCACTGGGACTGTCCAT (F) | 108 | D16501 (11) |
| GCACCACCATACTTGTTCCA (R) |
a
F, forward; R, reverse.