FIG. 2.

TSP mapping. (a and b) Primer extension with M. tuberculosis RNA isolated from a 48-h standing culture (lanes 1) and from aerobic culture (lanes 2); narK2 TSP was mapped with the primer narK2R3 and Rv1738 TSP was mapped with the primer Rv1738R. Dideoxy sequencing reactions using the same primers are shown to the left. (c) Alignment of narK2 and Rv1738 sequences from −40 to +20 bp (with respect to TSP). (d) Putative −10 and −35 promoter sequences of Rv1738 and narK2 which resemble SigC consensus sequences. The nucleotides which do not match the consensus sequence are shown in lowercase letters.