FIG. 4.
DevR∼P interacts with three binding sites in narK2-Rv1738 intergenic region. DNase I footprinting was performed with narK2-Rv1738 intergenic DNA (with the Rv1738 coding strand labeled with 32P) and either increasing concentrations of DevR∼P or the DevR D54V protein at highest concentration. D4 does not interact with DevR∼P. Bent arrows indicate the positions of the TSPs. The arrowhead indicates the hypersensitive sites (HS). Dideoxy sequencing reactions using the same primer and DNA template are also shown. The asterisk indicates the strand that is 32P labeled.