FIG. 7.

Wild-type promoter activity and effect of Dev box mutations on transcriptional activation of narK2 and Rv1738. GFP fluorescence (RFU/OD unit at 595 nm) of M. tuberculosis standing cultures after subtraction of background fluorescence derived from pFPV27. GFP fluorescence is constant in bacteria harboring the pFPV27 control vector. Percent expression with respect to the wild-type promoter is given in parentheses. Relative induction is a ratio of RFU/OD unit of standing cultures versus that of aerobic shaking cultures at 48 h (without subtracting background fluorescence derived from pFPV27). Shown are the average values of four experiments, each performed in triplicate. The number of RFU varied <15% between the experiments.