TABLE 1.
Plasmids used in this study
| Plasmid | Relevant feature(s)a | Reference or source |
|---|---|---|
| pSC-DevR | pGEX4T1 overexpressing DevR with a glutathione S-transferase N-terminal tag | 1 |
| pDevR D54V | pSC-DevR containing aspartic acid to valine mutation at amino acid residue 54 of DevR | K. Kaur and J. S. Tyagi |
| pFPV27 | E. coli mycobacterial shuttle plasmid with promoterless gfp; Kmr | 19 |
| pnarK2 | pFPV27 containing narK2 promoter (−220 to +57); Hygr | This study |
| p1738 | pFPV27 containing Rv1738 promoter (−203 to +74); Hygr | This study |
| pAmutD1 | pnarK2 containing mutated D1 DevR binding site; Hygr | This study |
| pAmutD2 | pnarK2 containing mutated D2 DevR binding site; Hygr | This study |
| pAmutD3 | pnarK2 containing mutated D3 DevR binding site; Hygr | This study |
| pAmutD1 D3 | pnarK2 containing mutated D1 and D3 DevR binding sites; Hygr | This study |
| pBmutD1 | p1738 containing mutated D1 DevR binding site; Hygr | This study |
| pBmutD2 | p1738 containing mutated D2 DevR binding site; Hygr | This study |
| pBmutD3 | p1738 containing mutated D3 DevR binding site; Hygr | This study |
| pBmutD1 D3 | p1738 containing mutated D1 and D3 DevR binding sites; Hygr | This study |
| pnarK2Δup | pFPV27 containing narK2 promoter (−121 to +57); Hygr | This study |
| pnarK2Δdn | pFPV27 containing narK2 promoter (−220 to +13); Hygr | This study |
| phspX | pFPV27 containing hspX promoter (−132 to +48); Hygr | D. K. Saini and J.S. Tyagi |
| p3134c-3 | pFPV27 containing Rv3134c promoter (−348 to +90); Hygr | 5 |
| psigA | pFPV27 containing sigA promoter (−238 to + 80); Hygr | N. K. Taneja and J.S. Tyagi |
a
The coordinates of the promoters (in parentheses) are with reference to the transcriptional start site except for the sigA promoter, which is with respect to translational start site.