TABLE 2.
Primers used in this study
| Primer | Sequence (5′ → 3′)a | Application |
|---|---|---|
| narK2F | GACACGATCCGGGGTCTCG | Reporter assay, gel shift assay, DNase I footprinting |
| narK2R | TCCCCTTTCCAGTGGCGACC | Reporter assay, gel shift assay, DNase I footprinting |
| narK2F1 | GGGGAATTCCGGACAGCCATGCGCTAACCC | Reporter assay |
| narK2R1 | GGGGAATTCCCCTCCGATAGGGCCTGAAGT | Reporter assay |
| narK2R3 | GTTCCACGCCCAGAAGTTGAC | Primer extension |
| Rv1738R | CGCCCGAGTCAATCCTTCGTGTTC | Primer extension |
| PD1 | ATGGCGCCGGCTCAGTTACCCCCGGAAGTCCCC | Mutational analysis |
| PD2 | CACTGCCCTGATTCGTCCGCCAGGGGC | Mutational analysis |
| PD3 | AGCCCTCCGATAGGGCCTGAAGTGGGGGCCACTGGGGTC | Mutational analysis |
a
EcoR1 sites are underlined, and mutated residues are in boldface.