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Sedimentation velocity analysis of TrwK. The sedimentation velocity profiles and distribution of sedimentation coefficients were obtained from experiments conducted either in HEPES buffer at pH 7.25 (A, B) or PIPES buffer at pH 6.45 (C, D). The labeled peaks in panels B and D correspond to monomers (a), dimers (b), trimers (c), and hexamers (d). OD, optical density [c(s)].
