TABLE 1.

Conjugation frequencies of R388 and trwK mutants in the presence of complementing proteins

Plasmids in donora	Variant of TrwK in donor	Conjugation frequenciesb
R388	TrwK	4 × 10−1
pSU4133	R388::ΔtrwK	1 × 10−6
pSU4133 + pET3a	None	4 × 10−7
pSU4133 + pET3a_trwK	TrwK	2 × 10−2
pSU4133 + pET3a_trwK_D654A	TrwK(D654A)	7 × 10−6

^aDonor cells (E. coli K12 strain DH5α) carrying the plasmids shown in the first column were mated with strain UB1637.

^bTransfer frequencies of transconjugants selected on streptomycin and trimethoprim plates.