Multiple sequence alignment of the α- and β-subunits of HQDO from P. fluorescens ACB. CLUSTAL W alignment of the amino acid sequences of HapC and HapD from P. fluorescens ACB and hypothetical proteins from Pseudomonas aeruginosa PA7 (gi 152988009 and gi 152987326), Burkholderia sp. strain 383 (gi 78063587 and gi 78063586), and Photorhabdus luminescens subsp. laumondii TTO1 (gi 37524165 and gi 37524166). Conserved residues are in dark boxes, and similar residues are in gray boxes. The amino acid sequences predicted by MALDI-TOF-TOF peptide mapping and sequencing are depicted on top of the CLUSTAL W alignment by asterisks. Related sequences of other Burkholderia species (B. cepacia AMMD [gi 115359956 and gi 115359957], Burkholderia ambifaria MC40-6 [gi 11869769 and gi 118697689], Burkholderia cenocepacia PC184 [gi 84353671 and gi 84353670], Burkholderia multivorans ATCC 17616[gi 118718179 and gi 118718178], Burkholderia phymatum STM815 [gi 118032827 and gi 118032828], and Burkholderia cenocepacia HI2424 [gi 116691528 and gi 116691529]) are not shown in the alignment but are very similar to the Burkholderia sp. strain 383 sequences.