FIG. 7.

Effect of oxygen on the synthesis of Tpx and Bcp in wild-type cells. (A) Growth curves of microaerobically (m; closed triangles) and aerobically (a; open triangles) grown wild-type C. jejuni used to measure Tpx and Bcp expression. (B) Coomassie blue-stained SDS-polyacrylamide gel showing normalized protein loadings of CEs from each time point for microaerobic (m) and aerobic (a) cells. (C and D) Immunoblots (upper panels) and corresponding densitometry histograms (lower panels; arbitrary units) of gels identical to panel B, using anti-Tpx (C) or anti-Bcp (D) as the primary antibody. Note that for the 24-h samples only the density of the major Tpx or Bcp band was measured.