TABLE 2.
Regulation of dapB transcription by lysine, arginine, and ArgP
| Strain | Relevant genotypea | β-Galactosidase activity (Miller units) inb:
|
|||
|---|---|---|---|---|---|
| Minimal glucose | Minimal glucose + lysine (10 mM) | Minimal glucose + arginine (10 mM) | Minimal glucose + arginine (10 mM) + lysine (10 mM) | ||
| JCP75 | Φ(dapBp[−118/+35]-lac) | 99 | 23 | 57 | 24 |
| JCP95 | Φ(dapBp[−118/+35]-lac) ΔargP::Camr | 21 | 20 | 21 | 20 |
| JCP76 | Φ(dapBp[−81/+35]-lac) | 10 | 11 | 10 | 11 |
| JCP96 | Φ(dapBp[−81/+35]-lac) ΔargP::Camr | 11 | 11 | 10 | 11 |
a
The extent of the DNA fragment carrying dapBp is indicated between brackets.
b
Cells were grown aerobically at 37°C in M63 glucose. Overnight cultures were diluted 100-fold in the same medium and grown to an optical density at 600 nm of 0.8.