TABLE 1.
Bacterial strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Relevant genotype or sequence (5′-3′) | Purpose | Source or reference |
|---|---|---|---|
| Strains | |||
| MR-1 | Wild type | ATCC | |
| ΔSO0166 | ΔgspD | D. Saffarini | |
| ΔSO0169 | ΔgspG | D. Saffarini | |
| ΔSO0169 complement | ΔgspG + pgspG | D. Saffarini | |
| MR-1 + pLS132 | Wild type + pomcA | This study | |
| MR-1 + pLS138 | Wild type + pmtrC | This study | |
| MR-1 + pLS146 | Wild type + pmtrC | This study | |
| MR-1 + pLS147 | Wild type + pomcA | This study | |
| ΔSO0166 + pLS132 | ΔgspD + pomcA | This study | |
| ΔSO0166 + pLS138 | ΔgspD + pmtrC | This study | |
| ΔSO0166 + pLS146 | ΔgspD + pmtrC | This study | |
| ΔSO0166 + pLS147 | ΔgspD + pomcA | This study | |
| ΔSO0169 + pLS132 | ΔgspG + pomcA | This study | |
| ΔSO0169 + pLS138 | ΔgspG + pmtrC | This study | |
| ΔSO0169 + pLS146 | ΔgspG + pmtrC | This study | |
| ΔSO0169 + pLS147 | ΔgspG + pomcA | This study | |
| Plasmids | |||
| pLS132 | Coding sequence for omcA | 25 | |
| pLS138 | Coding sequence for mtrC | 28 | |
| pLS146 | Coding sequence for the signal peptide of mtrB and the mature mtrC that lacks a lipid anchor | This study | |
| pLS147 | Coding sequence for the signal peptide of mtrB and the mature omcA that lacks a lipid anchor | 7 | |
| Primers | |||
| SO0166F | CAATAACACGCGTTAGGGG | Validation of ΔgspD | |
| SO0166R | CCTCGACTTGGGATACTTG | Validation of ΔgspD | |
| SO0169F | GTTACGCTCGCCCTCGG | Validation of ΔgspG | |
| SO0169R | TACGGTTTCATCGAGCAC | Validation of ΔgspG | |
| SO1776EF1 | CACCTAAGAAGGAGATATACATCCCATGAAATTTAAACTCAATTTGATCAC | Construction of pLS146 | |
| SO1776SR | ATTACCATCGCTTCCACCATCAGCAGCGACGGCCAAG | Construction of pLS146 | |
| SO1778EF3 | GGTGGAAGCGATGGTAATAAC | Construction of pLS146 | |
| SO1778ER3 | CTTGCAACAACCAGGGCAACATGCAGCAAGCTTGTCATCGTCATCCATTTTCACTTTAGTGTGATCTGC | Construction of pLS146 |