TABLE 2.
ORFs of a 14-kb DNA fragment of P. fluorescens ACB
| ORF | Frame | Position in sequence (nt) | Gene product
|
Function | Closest characterized/uncharacterized homolog(s)b
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| No. of residues | Mass (Da) | Description | GenBank accession no. | Sequence identity (%) | Reference | Organismc | ||||
| hapC | +3 | 360-857 | 165 | 17,899 | Hypothetical protein | ZP_01296719 | 78 | P. aeruginosa PA7 | ||
| hapD | +1 | 904-1929 | 341 | 38,345 | Hydroquinone dioxygenase | Hypothetical protein | ZP_01296720 | 80 | P. aeruginosa PA7 | |
| hapE | +1 | 2020-3483 | 487 | 52,400 | 4-Hydroxymuconic semialdehyde | p-Cumic aldehyde dehydrogenase | AAB62298 | 47 | 22 | P. putida F1 |
| dehydrogenase | Hypothetical protein | ZP_01296721, | 85 | P. aeruginosa PA7 | ||||||
| hapF | +3 | 3492-4559 | 355 | 37,612 | Maleylacetate reductase | Maleylacetate reductase | Q45072 | 56 | 20 | B. cepacia AC1100 |
| hypothetical protein | ZP_01296722 | 76 | P. aeruginosa PA7 | |||||||
| hapG | +1 | 4603-5478 | 291 | 32,159 | Hydroxyhydroquinone dioxygenase | Hydroxyquinol 1,2-dioxygenase | BAF44523 | 49 | 95 | Rhizobium sp. MTP-10005 |
| Hypothetical protein | ZP_01296723 | 67 | P. aeruginosa PA7 | |||||||
| hapH | +3 | 5475-5786 | 103 | 11,828 | Protein YciI family | Putative protein | ZP_01296724 | 72 | P. aeruginosa PA7 | |
| hapI | +3 | 5790-6179 | 129 | 13,866 | Ferredoxin | Chloroplast-type ferredoxin XylT | P23103, | 17 | 31 | P. putida |
| Hypothetical protein | ZP_01296725 | 58 | P. aeruginosa PA7 | |||||||
| hapB | +1 | 6169-7086 | 305 | 33,262 | 4-Hydroxyphenyl acetate esterase | Lipase | AAC38151 | 42 | 14 | Pseudomonas sp. strain B11-1 |
| Esterase/lipase | YP_299651 | 45 | R. eutropha JMP134 | |||||||
| hapA | +2 | 7139-9061 | 640 | 71,957 | 4-Hydroxyacetophenone | Steroid monooxygenase | JC7158 | 21 | 63 | R. rhodochrous |
| monooxygenase | Hypothetical protein | YP_639903 | 39 | Mycobacterium sp. strain MCS | ||||||
| orf10 | +3 | 9162-10094 | 310 | 35,222 | Regulatory protein (LysR family) | HTH-type transcript. regulator ptxR | P72131 | 24 | 30 | P. aeruginosa PA01 |
| Hypothetical protein | ZP_01296718 | 67 | P. aeruginosa PA7 | |||||||
| orf11 | −3 | 10166-11626 | 486 | 53,006 | Outer membrane channel protein | Outer membrane channel protein ttgF | CAB72260 | 55 | 64 | P. putida |
| Hypothetical efflux system | ZP_00898203 | 54 | P. syringae | |||||||
| orf12 | +2 | 11774-12382 | 202 | 22,221 | Regulatory protein (TetR family) | HTH-type transcriptional regulator srpR | Q9R9T9 | 57 | 92 | P. putida S12 |
| Hypothetical transcript. regulator, TetR | ZP_01499416 | 27 | B. phymatum STM815 | |||||||
| orf13 | +3 | 12450-13241 | 263 | 28,189 | Regulatory protein (Ic1R family) | HTH-type transcriptional regulator srpS | Q9R9U0 | 57 | 92 | P. putida S12 |
| Hypothetical regulatory protein | ZP_00898206 | 55 | P. putida F1 | |||||||
| orf14 | +1 | 13801-14736 | 311 | 34,623 | Efflux pump (EmrA family) | Multidrug resistance protein A | P27303 | 21 | 56 | E. coli |
| Hypothetical secretion protein | YP_574727 | 48 | C. salexigens | |||||||
ant, nucleotides.
b
Characterized homologs are shown in boldface.
c
R. eutropha, Ralstonia eutropha; P. syringae, Pseudomonas syringae; B. phymatum, Burkholderia phymatum; E. coli, Escherichia coli; C. salexigens, Chromohalobacter salexigens.