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. 2008 Jun 6;190(15):5353–5361. doi: 10.1128/JB.00181-08

FIG. 2.

FIG. 2.

The fur-fsl region and transcriptional analysis of fsl genes. (A) Representation of the fur-fsl region of the chromosome. The locations of primers used in RT-PCR are shown. (B) qPCR analysis of fslA and fslE expression in the Δfur mutant. cDNAs prepared from Schu S4 and from GR203 were tested for fslA and fslE expression relative to that of trpB as an internal standard. Reactions were run in triplicate, and results are represented as averages with standard deviations. Note that the y axis for fslA expression is a log scale. (C) RT-PCR to delineate the fsl operon. cDNA prepared from GR203 was used in PCR with primer pairs to detect transcription across genetic loci as follows: 109-112 for fur and fslA, 110-112 for fslA, 137-132 for fslAB, 182-119 for fslBC, 138-136 for fslCD, 178-165 for fslDE, 184-211 for fslEF, and 204-201 for fslF and FTT0023c. “−” represents negative controls, where reverse transcriptase was left out of the cDNA reaction; “+” indicates reactions with cDNA; and “g” represents reactions with gDNA as a template. “M” represents the 1-kb DNA ladder (Invitrogen).