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. 2008 Jun 6;190(15):5353–5361. doi: 10.1128/JB.00181-08

FIG. 3.

FIG. 3.

Western blotting with FslE antibodies. Whole-cell lysates normalized for cell density were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to a polyvinylidene difluoride membrane, and probed with polyclonal antiserum raised to recombinant FslE. The locations of the prestained standards run on the gel are indicated. The lysates are denoted by lanes: 1, GR203 (Δfur) grown in iron-replete CDM; 2, Schu S4 grown in iron-replete CDM; 3, Schu S4 grown in iron-limiting CDM; 4, GR211 (ΔfslE) grown in iron-limiting CDM; 5, vector (pFNLTP6gro-GFP)-transformed GR211 grown in iron-limiting CDM; 6, fslE+ plasmid (pGIR469)-transformed GR211 grown in iron-limiting CDM.