TABLE 1.
Bacterial strains and culture media
| Strain | Culture mediuma | Minimal biofilm medium | Source or reference |
|---|---|---|---|
| P. tunicata | VNSS (36) | 3M supplemented with 0.01% trehalose (43) | 26 |
| P. tunicata ΔAlpP mutant Smr Kmr | VNSS + Sm at 100 μg ml−1 + Km at 50 μg ml−1 | 3M supplemented with 0.01% trehalose (43) | 34 |
| M. mediterranea MMB-1 | Marine medium 2216 (Difco) | Marine minimal medium MN (25) | 48 |
| M. mediterranea SB1 lodA mutant Kmr | Marine medium 2216 + Km at 50 μg ml−1 (Difco) | Marine minimal medium MN (25) | 31 |
| C. violaceum | Luria-Bertani broth (Difco) | M9 (39) | UNSW culture collection (accession no. 040100) |
| C. violaceum CVMUR1 Kmr ΔNP_902938 | Luria-Bertani broth + Km at 40 μg ml−1 | M9 (39) | This study |
| C. crescentus CB15 | PYE: peptone, 2 g liter−1; yeast extract, 1 g liter−1; MgSO4·7H2O, 0.2 g liter−1 | M2 supplemented with 0.2% xylose (12) | ACMa (accession no. ACM 5171) |
| C. crescentus CAUMUR1 Smr ΔNP_419374 | PYE + Sm at 5 μg ml−1 | M2 supplemented with 0.2% xylose (12) | This study |
| E. coli DH5α | Luria-Bertani broth | 20 |
a
Sm, streptomycin; Km, kanamycin.
bACM, Australian Collection of Microorganisms.