FIG. 2.

Effects of glnA disruption on expression of lacZ fused to various regions upstream of degU. Cells grown overnight in glutamine-containing LB medium were inoculated into glutamine-containing Schaeffer's sporulation medium, and β-galactosidase activities were determined as described in Materials and Methods. Open circles, wild type; solid circles, glnA mutant. (A) Strains AY4450 and AY4450G; (B) AY4434 and AY4434G; (C) AY4407 and AY4407G; (D) AY9707 and AY9707G; (E) AY4407M and AY4407MG; (F) AY9734 and AY9734G; (G) AY9734M and AY9734MG; (H) AY3934 and AY3934G; (I) AY3920 and AY3920G; (J) AY5134 and AY5134G; (K) AY6234 and AY6234G; (L) AY8134 and AY8134G.