TABLE 1.
Bacterial strains used in this study
| Strain | Genotype or description | Reference or source |
|---|---|---|
| B. subtilis strains | ||
| CU741 | trpC2 leuC7 | 41 |
| AY741G | trpC2 leuC7 glnA::Bsr | This study |
| CU741T | trpC2 leuC7 tnrA::Cmr | This study |
| TT711 | trpC2 leuC7 degU::Cmr | 38 |
| HJS31 | ΔglnR57 | 32 |
| CU741SN | trpC2 leuC7 degS::Nmr | pDBNM1 × CU741 |
| CU741S | trpC2 leuC7 degS619; carries an in-phase deletion of degS (codons 11 to 170) | This study |
| AY101 | trpC2 leuC7 amyE::lacZ (no promoter) | pDLK2 × CU741 |
| AY4450 | trpC2 leuC7 amyE::degU-lacZ(1044-1350) (Nmr) | pAY4450 × CU741 |
| AY4450G | trpC2 leuC7 amyE::degU-lacZ(1044-1350) (Nmr) glnA::Bsr | AY741G × AY4450 |
| AY4434 | trpC2 leuC7 amyE::degU-lacZ(1044-1234) (Nmr) | pAY4434 × CU741 |
| AY4434G | trpC2 leuC7 amyE::degU-lacZ(1044-1234) (Nmr) glnA::Bsr | AY741G × AY4434 |
| AY4407 | trpC2 leuC7 amyE::degU-lacZ(1044-1207) (Nmr) | pA4407 × CU741 |
| AY4407G | trpC2 leuC7 amyE::degU-lacZ(1044-1207) (Nmr) glnA::Bsr | AY741G × AY4407 |
| AY4407T | trpC2 leuC7 amyE::degU-lacZ(1044-1207) (Nmr) tnrA::Cmr | CU741T × AY4407 |
| AY4407GT | trpC2 leuC7 amyE::degU-lacZ(1044-1207) (Nmr) glnA::BsrtnrA::Cmr | CU741T × AY4407G |
| AY4407U | trpC2 leuC7 amyE::degU-lacZ(1044-1207) (Nmr) degU::Cmr | TT711 × AY4407 |
| AY4407GU | trpC2 leuC7 amyE::degU-lacZ(1044-1207) (Nmr) glnA::BsrdegU::Cmr | TT711 × 4407G |
| AY9707 | trpC2 leuC7 amyE::degU-lacZ(1097-1207) (Nmr) | pAY9707 × CU741 |
| AY9707G | trpC2 leuC7 amyE::degU-lacZ(1097-1207) (Nmr) glnA::Bsr | AY741G × AY9707 |
| AY4407M | Same as AY4407 except for carrying sequence alterations | This study |
| AY4407MG | Same as AY4407G except for carrying sequence alterations | AY741G × AY4407M |
| AY9734 | trpC2 leuC7 amyE::degU-lacZ(1097-1234) (Nmr) | pAY9734 × CU741 |
| AY9734G | trpC2 leuC7 amyE::degU-lacZ(1097-1234) (Nmr) glnA::Bsr | AY741G × AY9734 |
| AY9734M | Same as AY9734 except for a mutation at the P3 promoter | This study |
| AY9734MG | Same as AY9734G except for a mutation at the P3 promoter | AY741G × AY9734M |
| AY3934 | trpC2 leuC7 amyE::degU-lacZ(1139-1234) (Nmr) | pAY3949 × CU741 |
| AY3934G | trpC2 leuC7 amyE::degU-lacZ(1139-1234) (Nmr) glnA::Bsr | AY741G × AY3934 |
| AY3920 | trpC2 leuC7 amyE::degU-lacZ(1139-1220) (Nmr) | pAY3920 × CU741 |
| AY3920G | trpC2 leuC7 amyE::degU-lacZ(1139-1220) (Nmr) glnA::Bsr | AY741G × AY3920 |
| AY5134 | trpC2 leuC7 amyE::degU-lacZ(1151-1234) (Nmr) | pAY5134 × CU741 |
| AY5134G | trpC2 leuC7 amyE::degU-lacZ(1151-1234) (Nmr) glnA::Bsr | AY741G × AY5134 |
| AY5134T | trpC2 leuC7 amyE::degU-lacZ(1151-1234) (Nmr) tnrA::Cmr | CU741T × AY5134 |
| AY5134GT | trpC2 leuC7 amyE::degU-lacZ(1151-1234) (Nmr) glnA::BsrtnrA::Cmr | CU741T × AY5134G |
| AY5134U | trpC2 leuC7 amyE::degU-lacZ(1151-1234) (Nmr) degU::Cmr | TT711 × AY5134 |
| AY5134GU | trpC2 leuC7 amyE::degU-lacZ(1151-1234) (Nmr) glnA::BsrdegU::Cmr | TT711 × AY5134G |
| AY5134S | trpC2 leuC7 amyE::degU-lacZ(1151-1234) (Nmr) degS619 | AY5134 × CU741S |
| AY5134GS | trpC2 leuC7 amyE::degU-lacZ(1151-1234) (Nmr) glnA::BsrdegS619 | AY741G × AY5134S |
| AY5134HY | trpC2 leuC7 amyE::degU-lacZ(1151-1234) (Nmr) degS200(Hy) | This study |
| AY6234 | trpC2 leuC7 amyE::degU-lacZ(1162-1234) (Nmr) | pAY6234 × CU741 |
| AY6234G | trpC2 leuC7 amyE::degU-lacZ(1162-1234) (Nmr) glnA::Bsr | AY741G × AY6234 |
| AY6234U | trpC2 leuC7 amyE::degU-lacZ(1162-1234) (Nmr) degU::Cmr | TT711 × AY6234 |
| AY8134 | trpC2 leuC7 amyE::degU-lacZ(1181-1234) (Nmr) | pAY8134 × CU741 |
| AY8134G | trpC2 leuC7 amyE::degU-lacZ(1181-1234) (Nmr) glnA::Bsr | AY741G × AY8134 |
| AYDS11 | trpC2 leuC7 amyE::degS-lacZ | pDegS12 × CU741 |
| AYDS11G | trpC2 leuC7 amyE::degS-lacZ glnA::Bsr | AY741G × AYDS11 |
| AYDS11U | trpC2 leuC7 amyE::degS-lacZ degU::Cmr | TT711 × AYDS11 |
| E. coli strain | ||
| JM103 | Δlac-pro thi rpsL supE sbcB hsdR4 F′ [traD36 proAB+lacIqlacZΔM15] | 45 |