TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| SM10λpir | thi-1 leu tonA lacY supE recA::RP4-2-Tc::Mu λpir Kmr | 28a |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| 5131 | Porcine ExPEC O115:K“V165”:F165 | 12a |
| K2 | 5131 Δpst::kan Kmr | This study |
| CK2 | K2 + pAN92; Kmr Cmr | This study |
| χ7122 | APEC O78:K80:H9 gyrA Nalr | 33 |
| K3 | χ7122 ΔpstCAB::kan | 25 |
| CK3 | K3 + pAN92 | 25 |
| Plasmids | ||
| pCR2.1 | Cloning vector | Invitrogen |
| pKNG101 | Suicide vector; SmrsacB | 23a |
| pKNG800K | pKNG101::pstCAB::kan; sacB Smr Kmr | 25 |
| pAN92 | pACYC184::pst operon; Cmr | 23 |
a
Resistance abbreviations: Kmr, kanamycin; Cmr, chloramphenicol; Smr, streptomycin; Nalr, nalidixic acid, and Tetr, tetracycline.