TABLE 1.
Strains used in this study
| Strain | Genotype | Functional category of deleted/disrupted gene(s) | Source or reference |
|---|---|---|---|
| ATCC 21332 | Wild type | 21 | |
| JJM405 | ΔdhbF2::kan | Dimodular nonribosomal peptide synthetase for BB assembly (complete second module deleted) | 20 |
| BMM100 | ΔdhbC::erm | Isochorismate synthase | 21 |
| BMM141 | ymfE::pMUTIN | MFS-type transport | This studya |
| BMM142 | mta::pMUTIN | Transcriptional activator of multidrug-efflux transporters; disruption leaves residues 1 to 99 of the N terminal DNA binding domain of Mta (N-Mta) under native control | This study |
| BMM143 | Δmta::spc | Transcriptional activator of multidrug-efflux transporters | This study |
| BMM150 | ΔywjA::erm | ABC-type transport (Fur target) | This study |
| BMM161 | yttB::pMUTIN | MFS-type transport | This study |
| BMM162 | yubD::pMUTIN | MFS-type transport | This study |
| BMM163 | ygaD::pMUTIN | ABC-type transport | This study |
| BMM164 | yvmA::pMUTIN | MFS-type transport | This study |
| BMM165 | yvkA::pMUTIN | MFS-type transport | This study |
| BMM166 | yvaE::pMUTIN | DMT-type transport | This study |
| BMM167 | ywfF::pMUTIN | MFS-type transport | This study |
| BMM168 | yusP::pMUTIN | MFS-type transport | This study |
| BMM169 | mrpF::pMUTIN | Efflux for pH homeostasis | This study |
| BMM170 | ykuC::pMUTIN | MFS-type transport | This study |
| BMM171 | ykkD::pMUTIN | DMT-type transport | This study |
| BMM172 | ykkC::pMUTIN | DMT-type transport | This study |
| BMM173 | yeaB::pMUTIN | CDF-type transport | This study |
| BMM174 | yerP::pMUTIN | RND-type transport | This study |
| BMM175 | yvqJ::pMUTIN | Macrolide efflux | This study |
| BMM176 | ebrA::pMUTIN | DMT-type transport | This study |
| BMM177 | ebrB::pMUTIN | DMT-type transport | This study |
| BMM178 | ymfD::pMUTIN | MFS-type transport | This study |
| BMM179 | yitG::pMUTIN | MFS-type transport | This study |
| BMM180 | yitZ::pMUTIN | MFS-type transport | This study |
| BMM181 | tatAC::pMUTIN | Twin arginine-dependent transport | This study |
| BMM182 | tatAD::pMUTIN | Twin arginine-dependent transport | This study |
| BMM183 | tatAY::pMUTIN | Twin arginine-dependent transport | This study |
| BMM184 | tatAC::pMUTIN | Twin arginine-dependent transport | This study |
| BMM185 | tatCY::pMUTIN | Twin arginine-dependent transport | This study |
| BMM186 | yfmI::pMUTIN | Macrolide efflux | This study |
| BMM187 | ywjA::pMUTIN | ABC-type transport (Fur target) | This study |
| BMM188 | yhcA::pMUTIN | MFS-type transport | This study |
| BMM189 | yfiS::pMUTIN | MFS-type transport | This study |
| BMM190 | ybbB::pMUTIN | Transcriptional activator for Fe-BB uptake (Fur target) | This study |
| BMM191 | yfiB::pMUTIN | ABC-type transport | This study |
| BMM192 | yfiC::pMUTIN | ABC-type transport | This study |
| BMM193 | blt::pMUTIN | MFS-type transport | This study |
| BMM194 | yceJ::pMUTIN | MFS-type transport | This study |
| BMM195 | ycnB::pMUTIN | MFS-type transport | This study |
| BMM196 | yfiU::pMUTIN | MFS-type transport | This study |
| BMM197 | yfkF::pMUTIN | MFS-type transport | This study |
| BMM198 | yfkL::pMUTIN | MFS-type transport | This study |
| BMM199 | yhjO::pMUTIN | MFS-type transport | This study |
| BMM200 | yqjV::pMUTIN | MFS-type transport | This study |
| BMM201 | yuxJ::pMUTIN | MFS-type transport | This study |
| BMM202 | ycbK::pMUTIN | DMT-type transport | This study |
| BMM203 | yjbB::pMUTIN | Macrolide efflux | This study |
| BMM204 | yrhP::pMUTIN | Putative lysine exporter | This study |
| BMM205 | yheH::pMUTIN | ABC-type transport | This study |
| BMM206 | yknU::pMUTIN | ABC-type transport | This study |
| BMM207 | yknV::pMUTIN | ABC-type transport | This study |
| BMM208 | yvcC::pMUTIN | ABC-type transport | This study |
| BMM209 | yhbJ::pMUTIN | Unknown type of multidrug efflux | This study |
| BMM210 | ywfA::pMUTIN | MFS-type transport | This study |
| BMM211 | ywcC::pMUTIN | Putative transcriptional regulator | This study |
| BMM212 | ysbA::pMUTIN | Putative transcriptional regulator | This study |
| BMM213 | yncC::pMUTIN | MFS-type transport | This study |
| BMM214 | ywbG::pMUTIN | Predicted exporter | This study |
a
All of the donor strains used in this study for transformation into the ATCC 21332 background were obtained from the BFA mutant library (18).