TABLE 2.
Oligonucleotide primers used in this study
| Primer | Nucleotide sequence (5′ to 3′) | Restriction site |
|---|---|---|
| PBMrIRPi | AGACCGGGGACTTATCAGCC | |
| PBMrTn1 | CTAGCGACGCCATCTATGTG | |
| TMr_1 | TGCATTTAATACTAGCGACGCCATCTATGTGTC | |
| TMr_4 | GGATCCATTCGCGTCAATTCGAGGGG | |
| PpsaB-1-lacZ | CGGAATTCTTCCAAGTTTTTTACACTTG | EcoRI |
| PpsaB-2-lacZ | CGGGATCCATTGTTGGTCCATGGAGCAC | BamHI |
| pspr1945-1 | CGGAATTCCCTTCAAATTTTAAGTCC | EcoRI |
| pspr1945-2 | CGGGATCCGTTAATGATAATATTGTAG | BamHI |
| PpcpA_f_2 | CGGAATTCTAATTTCTTTTTTAACCCAC | EcoRI |
| PpcpA_f_3 | CGGAATTCGTGGGTTAATTTTCCTTGAC | EcoRI |
| PpcpA_f_3a | CGGAATTCTAAAAAAGAAATTAAAGTGG | EcoRI |
| PpcpA_f_4 | CGGAATTCAATGTACTACTCTATTCTAC | EcoRI |
| PpcpA_rev_1 | CGGGATCCAGGATTGGTTCATTAGGGAC | BamHI |
| PpcpA_rev_3 | CGGGATCCGTCAAGGAAAATTAACCCAC | BamHI |
| PpcpA_rev_4 | CGGGATCCCTAGTAGAATAGAGTAGTAC | BamHI |
| marR-lacZ1 | CGGAATTCGCTATTTTCGTCATATCC | EcoRI |
| marR-lacZ2 | CGGGATCCCATTTTAGATAGTCTTCTTTG | BamHI |
| marR-del1 | TGCTCTAGACAATTGCCCACCAGTCCCG | XbaI |
| marR-del2 | TTCTTTGTTTGGGGTCATTC | |
| marR-del3 | GACCCCAAACAAAGAAGTCGAGAAAATCAACTAAT | |
| marR-del4 | GAAGATCTCTTTTGTCAGCTGAACGA | BglII |
| PprtA-1 | CGGAATTCTAGCGCTGATATTTCATC | |
| PprtA-2 | CGGGATCCGTTAGTGACAATACTGTG | |
| spr0561-1 | CGGAATTCGATACGGGAGAGGTAAGTG | EcoRI |
| spr0561-2 | CGGGATCCGAAATTGTCTCATCACCTC | BamHI |
| PSpr183-1.2 | CGGAATTCCAACCTAAGGTGATTGTGG | EcoRI |
| PSpr183-2.2 | CGGGATCCGAATTTCTGTAATAATTCGC | BamHI |
| Pspr0276-1 | CGGAATTCGAATTAGAATTATTGTGAG | BamHI |
| Pspr0276-2 | CGGGATCCGAGCAGCAACAGCACCACC | XbaI |
| Ery-for | TAACGATTATGCCGATAACT | |
| Ery-rev | GCATGCATCGATTAGATCTC | |
| psaCA-KO-1 | AAATCCTTACACCGAATTGC | |
| psaCA-KO-2 | GAGATCTAATCGATGCATGCTTCTGCAATCATAGGTCACCTCC | |
| psaCA-KO-3 | AGTTATCGGCATAATCGTTAGACAAGATTGCTGAAGGATTGG | |
| psaCA-KO-4 | TGCGCGTGCTAATAGGTGCC | |
| P-pcpA-box1 | CATGTCTTTTTTAACACGGGTTAAAAAAGAAATTAAAGTGGGTTAATTTTCCTTGACTTAAAATTTAA | |
| P-pcpA-box2 | CTAGTTAAATTTTAAGTCAAGGAAAATTAACCCACTTTAATTTCTTTTTTAACCCGTGTTAAAAAAGA | |
| PcpA_mut1.1 | GATTTCTTTTTTAACCCGTG | |
| PcpA_mut1.2 | AAAGTGGGTTAATTTTCCTTG | |
| PpcpA_mut2.1 | GTAATTTCTTTTTTAACCCGTG | |
| PpcpA_mut2.2 | AGTGGGTTAATTTTCCTTGAC | |
| PpcpA_mut3.1 | GCAATTTCTTTTTTAACCCGTG | |
| PpcpA_mut3.2 | AGTGGGTTAATTTTCCTTGAC | |
| Spr1480OX-2 | TGCTCTAGATTAGTTGATTTTCTCGACATAG | XbaI |
| spr1480-OX-new | CGAGCCATCATGAAACATCATCATCATCATCATACCCCAAACAAAGAAGACTATC | RcaI |
| Spr1480-Ctermstrep_OX | TGCTCTAGATTATTTTTCAAATTGTGGATGGCTCCAAGCGCTGTTGATTTTCTCGACATAGAGTTG | XbaI |
| Spr1480OX-1 | CGAGCCATCATGACCCCAAACAAAGAAGAC | RcaI |