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. 2008 May 30;190(15):5382–5393. doi: 10.1128/JB.00307-08

TABLE 5.

β-Galactosidase activities of the wild-type D39 and the ΔpsaR, ΔpsaCA, and ΔczcD strains, all containing the PpcpA-lacZ transcriptional fusion, grown in GM17 and GM17chel supplemented with metal ionsa

Medium and addition(s) (mM) Mean (SD) β-galactosidase activity (Miller units)b in:
Wild type ΔpsaR mutant ΔpsaCA mutant ΔczcD mutant
GM17
    None 6 (3) 1,204 (67) 594 (55) 18 (5)
    Zn2+ (0.1) 17 (4) ND ND 69 (12)
    Zn2+ (0.2) 66 (21) 1,102 (277) ND ND
    Zn2+ (0.4) 417 (113) 1,189 (115) 446 (37) ND
    Zn2+ (0.4) + Mn2+ (0.01) 33 (12) 1,119 (113) 237 (22) ND
    Zn2+ (0.4) + Mn2+ (0.05) 11 (4) 1,219 (203) 7 (4) ND
    Zn2+ (0.4) + Fe2+ (0.05) 269 (22) ND ND ND
GM17chel
    None 315 (47) 1,217 (222) ND ND
    Zn2+ (0.2) 424 (35) 1,260 (102) ND ND
    Mn2+ (0.05) 6 (2) 1,315 (78) ND ND
a

The wild-type D39 and the ΔpsaR, ΔpsaCA, and ΔczcD strains are strains RW102, RW107, RW114, and RW122, respectively.

b

Values are from three experiments. ND, not determined.

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