TABLE 5.
β-Galactosidase activities of the wild-type D39 and the ΔpsaR, ΔpsaCA, and ΔczcD strains, all containing the PpcpA-lacZ transcriptional fusion, grown in GM17 and GM17chel supplemented with metal ionsa
| Medium and addition(s) (mM) | Mean (SD) β-galactosidase activity (Miller units)b in:
|
|||
|---|---|---|---|---|
| Wild type | ΔpsaR mutant | ΔpsaCA mutant | ΔczcD mutant | |
| GM17 | ||||
| None | 6 (3) | 1,204 (67) | 594 (55) | 18 (5) |
| Zn2+ (0.1) | 17 (4) | ND | ND | 69 (12) |
| Zn2+ (0.2) | 66 (21) | 1,102 (277) | ND | ND |
| Zn2+ (0.4) | 417 (113) | 1,189 (115) | 446 (37) | ND |
| Zn2+ (0.4) + Mn2+ (0.01) | 33 (12) | 1,119 (113) | 237 (22) | ND |
| Zn2+ (0.4) + Mn2+ (0.05) | 11 (4) | 1,219 (203) | 7 (4) | ND |
| Zn2+ (0.4) + Fe2+ (0.05) | 269 (22) | ND | ND | ND |
| GM17chel | ||||
| None | 315 (47) | 1,217 (222) | ND | ND |
| Zn2+ (0.2) | 424 (35) | 1,260 (102) | ND | ND |
| Mn2+ (0.05) | 6 (2) | 1,315 (78) | ND | ND |
a
The wild-type D39 and the ΔpsaR, ΔpsaCA, and ΔczcD strains are strains RW102, RW107, RW114, and RW122, respectively.
b
Values are from three experiments. ND, not determined.