FIG. 3.

(A) GC chromatograms of TMS derivatives obtained from caldarchaeols corresponding to HPLC peaks shown in Fig. 2. (B) Structures corresponding to caldarchaeols shown in panel A. Caldarchaeols collected from the HPLC peaks in Fig. 2 were decomposed to biphytanyl alcohols and then converted to their TMS derivatives. TMS-biphytanyl alcohols were analyzed by GC using an HP-5ht capillary column (0.3 mm inside diameter by 30 m; thickness of film [df] = 0.1 μm) at a temperature gradient of 200°C to 350°C with an incremental step of 10 degrees/min and monitored with a flame ionization detector. The number of cyclopentane rings in the biphytanyl chains shown in the GC chart was identified previously (26).