TABLE 1.

Composition of polar lipids in T. acidophilum HO-62^a

Growth conditionb	% (by wt) of total polar lipids (mean ± SD)c
	GuCGu	GuCGp	CGp + AGp	GuC	MGuC	MGuCGu	DMGuC	TMGlC	MGuCGp	DMGuCGp	TMGuCGp
pH
3.0	6.9 ± 1.7	71.2 ± 3.0	ND	7.8 ± 1.4	ND	ND	ND	ND	3.3 ± 0.8	7.4 ± 2.6	3.3 ± 3.0d
2.4	6.5 ± 0.7	69.8 ± 1.8	ND	7.6 ± 0.4	ND	ND	ND	ND	4.1 ± 1.0	7.0 ± 2.1	4.9 ± 2.5
1.8	4.7 ± 1.7	61.9 ± 3.7	2.6 ± 0.8	9.5 ± 2.6	2.0 ± 0.6	2.0 ± 0.7	2.4 ± 0.8	0.8 ± 0.3	5.5 ± 0.9	6.3 ± 1.4	2.3 ± 2.1d
1.2	3.1 ± 0.8	36.9 ± 10.5	1.0 ± 1.7	23.4 ± 3.6	6.1 ± 1.9	3.1 ± 0.4	8.1 ± 3.1	1.7 ± 0.2	6.8 ± 0.5	5.6 ± 0.7	4.3 ± 2.1
Temp (°C)
45	6.2 ± 2.0	63.9 ± 7.3	2.8 ± 0.3	10.9 ± 1.7	1.2 ± 1.1d	1.0 ± 0.9d	0.9 ± 0.9d	0.2 ± 0.4d	6.8 ± 2.0	4.5 ± 2.2	1.8 ± 0.5
50	4.7 ± 0.9	64.4 ± 2.1	2.9 ± 0.4	8.9 ± 1.0	0.9 ± 0.8d	1.5 ± 0.1	1.5 ± 0.1	0.9 ± 1.1d	6.2 ± 0.6	4.9 ± 0.6	3.3 ± 1.3
55	4.7 ± 1.7	61.9 ± 3.7	2.6 ± 0.8	9.5 ± 2.6	2.0 ± 0.6	2.0 ± 0.7	2.4 ± 0.8	0.8 ± 0.3	5.5 ± 0.9	6.3 ± 1.4	2.3 ± 2.1d
60	3.4 ± 0.5	54.1 ± 4.1	4.5 ± 0.6	16.0 ± 3.7	4.1 ± 0.6	2.1 ± 0.5	5.0 ± 0.7	0.9 ± 0.7d	2.9 ± 2.5d	6.7 ± 0.2	0.4 ± 0.7d

^aEach value indicates the weighted percentage of each component with respect to the total weight of polar lipids quantified by ELSD-HPLC. Each datum is an average of three independent cultures, consisting of means ± standard deviations (SD). The total amount of polar lipid analyzed in each measurement varied from 20 to 40 μg.

^bT. acidophilum HO-62 was grown at different pHs at 55°C (Temp) and at pH 1.8 at different temperatures (pH).

^cND, all estimates in three independent cultures had values lower than that of the detection limit (less than 1.4% [weighted %]) for GuCGp.

^dOne or two data were below the detection limit and were assumed to be 0 for the calculation of an average.