FIG. 2.

Contribution of the gdpS gene to biofilm development in S. epidermidis CSF41498. (A) Biofilm phenotypes of S. epidermidis CSF41498 and GDPS1 (gdpS::Em^r) strains complemented with pLI50 (control) or pSEGP5 (S. epidermidis gdpS) in static 96-well plate assays. Strains were grown overnight at 37°C in BHI broth and BHI-NaCl broth supplemented. Biofilm formation was measured at least three times, and standard deviations are indicated. (B) Biofilm development by S. epidermidis CSF41498 pLI50, CSF41498 pSEGP5 (gdpS), GDPS1 (gdpS::Em^r) pLI50, and GDPS1 pSEGP5 (gdpS) strains using the Biosurface Technologies flow system. Strains were grown for 24 h at 37°C in BHI-NaCl broth, and the flow cells were photographed after 24 h. 41498, CSF41498.