TABLE 2.
Oligonucleotide primers used in this study
| Target gene (use) | Primer name | Primer sequence (5′-3′) |
|---|---|---|
| gyrB (RT-PCR) | GYRB1 | TTATGGTGCTGGACAGATACA |
| GYRB2 | CACCGTGAAGACCGCCAGATA | |
| 16S rRNA (RT-PCR) | 16Sfor | CATTTCACCGCTACACATGG |
| 16SRev | GAAAGCCACGGCTAACTACG | |
| icaA (RT-PCR) | KCA1 | AACAAGTTGAAGGCATCTCC |
| KCA2 | GATGCTTGTTTGATTCCCT | |
| icaR (RT-PCR) | icaRFor2 | ATCCAAAGCGATGTGCGTAG |
| icaRRev2 | TCCATTGACGGACTTTACCAG | |
| sarA (RT-PCR) | SarAFor | TCAGCTTTGAAGAATTTGCAG |
| SarARev | TCTTTCATCGTGTTCATTACGTTT | |
| asp23 (RT-PCR) | Asp23For | CATGAAAGGTGGCTTCACAG |
| Asp23Rev | CATTACGTCGTCAACTTGCAT | |
| luxS (RT-PCR) | SEluxSFor | TTCGTTTCAAACAGCCCAAT |
| SEluxRev | CTGGGACTTCGCTAGCATTT | |
| gdpS (RT-PCR) | SEdgcSFor | ATCTGTCATGGTGGCAGGTA |
| SEdgcSRev | CGATAAAAGCAGCCGTGAGT | |
| tcaR (RT-PCR) | SetcaRFor | TGGCATATCAGCTGAGCAAT |
| SEtcaRRev | TGGCTCATAATCGCTTTTCTC | |
| S. epidermidis gdpS | SEgmp1 | TGAATAAGTTGTACCTCACGCTATG |
| SEgmp2 | TCTCATCACCTCCGAAGCTA | |
| S. aureus gdpS | SAgmp1 | TGGTGTTATCGTCAAACTGACA |
| SAgmp2 | AACCTCCACTTAACTATACGG | |
| gdpS (ΔE270 and ΔE271 mutagenesis) | SEdgcSdel_1 | TTTAGAAACGGTGGCTTTTCTGTTGTAATA |
| SEdgcSdel_2 | TATTACAACAGAAAAGCCACCGTTTCTAAA | |
| S. epidermidis gdpS GGDEF domain | SE0528F | GCTCTAGATCAGCAATAACATTCGTTG |
| SE0528R | CCGAAGCTTTTATAATTTGACAATAGGATT | |
| gdpS transmembrane domain only | SEgmp1 | TGAATAAGTTGTACCTCACGCTATG |
| SESDM-STOP2 | TACCTAGACCTGTAAGTCAATCGTATTTATCTT | |
| S. epidermidis rsbV | SEsigB1 | TCTTGAGCTTGGCTATCTTCG |
| SEsigB2 | CGTTTGAACCGTGTTGTTGA | |
| S.epidermidis sigB locus | SEsigBoperon1 | TCACCAGTTCAAGGGTCTGA |
| SEsigBoperon2 | TCTTTGGAGCTTCGTCTGTG | |
| S. epidermidis gdpS GGDEF domain | SE0528F | GCTCTAGATCAGCAATAACATTCGTTG |
| SE0528R | CCGAAGCTTTTATAATTTGACAATAGGATT | |
| S. aureus gdpS GGDEF domain | SA0701F | GCTCTAGATCAGCGATAACATTTGTCG |
| SA0701R | CCGAAGCTTTTATAAATTGATAATAGGG | |
| S. aureus gdpS (full-length) | SA0701FL | GCTCTAGATTCGAAGCATTTATATACAAT |
| SA0701R | CCGAAGCTTTTATAAATTGATAATAGGG | |
| S. epidermidis gdpS (ORF only) | CN-SE0528F | CCTATGCATTTGAAGCTATCATATATAAC |
| CN-SE0528R | CGGGATCCTAGCTTGATTATAATTTGAC | |
| Synechocystis sp. strain PCC6803 slr1143 | CN-1143F | CCAATGCATCAGCTAAATTACCGC |
| CN-1143R | CGGGATCCTTATTCTGCCAGTTG | |
| E. coli yeaP | CN-YeaPF | CCAATGCATCGCACTATTTTTATTACATTC |
| CN-YeaPR | CGGGATCCTCAGGAATGTAGCGC |