CGH and adenylate cyclase toxin locus in strains RB50 and 253. CGH analyses of whole genome (A) or cya-related and surrounding genes (B) between strains RB50 and 253. The x axis indicates the order of genes along the B. bronchiseptica 5.3-megabase (Mb) chromosome. The y axis indicates the fold change in hybridization (FCH) for each gene. Negative-fold-change values indicate the decreased hybridization of strain 253 genomic DNA compared to that of strain RB50, while positive-fold-change values indicate increased hybridization. Genes of interest have been labeled with corresponding underscores. HP, hypothetical protein; Φ, phage-related gene; wbm, O-antigen-related genes; cyaA, adenylate cyclase toxin gene. Error bars represent the mean ± the standard error. Marks under B designate if genes upstream, within, and downstream of the cya operon could (+) or could not (−) be PCR amplified from strains RB50 or 253.