TABLE 1.
Genotypes/phenotypes of bacterial strains and plasmids used in this work
| Strain or plasmid | Genotype/phenotype | Source or reference |
|---|---|---|
| Bacterial strains | ||
| E. coli strains | ||
| VCS257 | DP50 sup F[supE44 supF58 hsd53(rB mB) dapD8 lacY1 glnV44 Δ(gal-uvrB)47 tyrT58 gyrA29 tonA53Δ (thyA57)] | Stratagene |
| BL21(DE3) | F−ompT rB mB | Novagen |
| DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Promega |
| H1594 | araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR aroB fiu::Mud1X (Ampr Strr) | 20 |
| H1875 | araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR aroB cir::Mud1X fepA::Tn10 (Ampr Tetr Strr) | 20 |
| H1876 | araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR aroB fiu::Mud1X cir fepA::Tn10 (Ampr Tetr Strr) | 20 |
| H5311 | H1143 derivative; araD139 Δ(argF lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR aroB entC::Mud1X | 19; K. Hantke lab collection |
| K12 entB | entB; obtained by gene replacement with Kan (Kanr) | 27 |
| ER1100A | entF; obtained by gene replacement with Chl (Chlr) | 38 |
| ER1300H | entF; ER1100A derivative sensitive to Chl | 38 |
| S. enterica strain | ||
| LT2 | Also known as Salmonella enterica serovar Typhimurium LT2; prototroph | S. Maloy lab collection |
| Plasmids | ||
| pJAM434 | Moderately low production of MccE492 (Ampr) | 45 |
| np133 | pJAM434 mceC::Tn5 (Ampr Kanr) | 24 |
| np205 | pJAM434 mceI::Tn5 (Ampr Kanr) | 24 |
| np221 | pJAM434 mceJ::Tn5 (Ampr Kanr) | 24 |
| np220 | pJAM434 mceA::Tn5 insertion in amino acid 43 (Ampr Kanr) | This work |
| pJEM15 | Overproduces MccE492 (Ampr) | 45 |
| npB4 | pJEM15 mceC::Tn5 insertion in amino acid 60 (Ampr Kanr) | This work |
| p157 | mceB (immunity) cloned into pT7-7 (Ampr) | 25 |
| pIroB | iroB of S. enterica cloned into pACYC184 (Chlr) | This work |
| pER311 | entF cloned into pET28-a with a six-His tag at the C terminus (Ampr) | 38 |
| pER307A | Domains A-PCP-TE (between amino acids 437 and 1293) of EntF cloned into pET28-a with a six-His tag at the C terminus (Ampr) | C.T. Walsh lab collection |
| pER304A | Domains A-PCP (between amino acids 437 and 1045) of EntF cloned into pET28-a with a six-His tag at the C terminus (Ampr) | C.T. Walsh lab collection |
| pACYC-EntF | entF cloned into pACYC184 as it is in pER311 (Chlr) | This work |
| pACYC-A-PCP-TE | Domains A-PCP-TE of EntF cloned into pACYC184 as in pER307A (Chlr) | This work |
| pACYC-A-PCP | Domains A-PCP of EntF cloned into pACYC184 as in pER304A (Chlr) | This work |
| pACYC-A | Domain A (between amino acids 437 and 968) of EntF cloned into pACYC184 (Chlr) | This work |
| pACYC-EntF-E750A | entF with the E750A mutation cloned into pACYC184 (Chlr) | This work |