TABLE 2.
Complementation of antibacterial activity in S. enterica and E. coli/pIroB of a mutant in the mceC maturation genea
| Plasmid | Complementation in host
|
|
|---|---|---|
| E. coli | S. enterica | |
| pJAM434 (wild type) | + | +++ |
| np133 (mceC) | − | ++ |
| np205 (mceI) | − | − |
| np221 (mceJ) | − | − |
| np220 (mceA) | − | − |
| npB4 (mceC) + pACYC184 | − | ND |
| npB4 (mceC) + pIroB | + | ND |
a
Aliquots (3 μl) of liquid cultures of E. coli VCS257 or S. enterica LT2 carrying the indicated plasmids were seeded on a lawn of sensitive cells as described in the legend to Fig. 1, and the activity was expressed as low (+), medium (++), or high (+++) (see Materials and Methods). ND, not determined. pACYC184 was the vector used to clone iroB.