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. 2008 May 14;82(15):7432–7442. doi: 10.1128/JVI.00379-08

FIG. 1.

FIG. 1.

(A) Schematic showing the incorporation of a BAC plasmid into the PacI site created within the intergenic region of the gB and UL26 genes of BHV-1 genome. (B) HindIII restriction profile analysis of wt BHV-1, BHV-1 BAC recombinant virus prior to propagation in E. coli, pBHV-1 BAC clones 1 and 2 isolated from E. coli, vBHV-1 BAC (reconstituted by cotransfection of pBHV-1 BAC clone 1 into MDBK cells), and BAC-excised vBHV-1lox DNA (reconstituted by cotransfection of pBHV-1 BAC DNA and pCREin into MDBK cells).