FIG. 2.

Identification and characterization of BHV-1gEAm453 virus. (A) Immunoblot analysis of cell lysates with BHV-1gE peptide-specific rabbit polyclonal antibody (12). Cells were either mock infected or infected with vBHV-1gEAm453R (rescued), vBHV-1gEAm453, and wt BHV-1 viruses. (B) Immunoblot analysis of identical Western blots containing lysates of partially purified BHV-1 gEΔ, vBHV-1gEAm453, vBHV-1gEAm453R, or wt BHV-1 virions with either anti-gE peptide rabbit antibody (as described for panel A), a cocktail of rabbit anti-gE peptide-specific and gI-specific polyclonal antibodies, or anti BHV-1 gC-specific monoclonal antibody F2 (11).